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00.092.0.81.005. Tobacco stunt virus

Cite this publication as: ICTVdB Management (2006). 00.092.0.81.005. Tobacco stunt virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Hiroshima Prefecture; Japan.

Host of Isolate and Habitat Details
Source of isolate: Nicotiana tabacum.

Natural host and symptoms
Nicotiana tabacum — stunting, vein necrosis, necrotic leaf spotting.

Reference to Isolation Report
Hidaka (1950).

Classification

This is a description of a plant virus at the species level.

ICTVdB Virus Code: 00.092.0.81.005. Virus accession number: 92081005. Obsolete virus code: 79.0.P.3.001; superceded accession number: 790p3001.
NCBI Taxon Identifier NCBI Taxonomy ID: 293544.

Name, Synonyms and Lineage

ICTV approved acronym: TStV. Virus is a tentative member of the genus 00.092.0.01.. Varicosavirus; not assigned to a family.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped. Capsid/nucleocapsid is elongated with helical symmetry. The capsid is rod-shaped, straight with a clear modal length with a length of 300 and 340 nm and a width of 18 nm. Axial canal is distinct; 3.5 nm in diameter. Basic helix is obvious. Pitch of helix is 5 nm.

Electron microscopic preparation and references: Virus preparation contains few virions. Fixation with glutaraldehyde necessary. Reference for electron microscopic methods: Kuwata and Kubo (1981).

Physicochemical and Physical Properties

The density of virions is 1.27 in Cs2SO4. The thermal inactivation point (TIP) is at 40-50°C (sap transmissible only after addition of reducing agents such as 0.01M Na2SO3). The longevity in vitro (LIV) is 1 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 3-4.

Nucleic Acid

The genome is segmented; bipartite, segements are distributed among 2 particle types of linear, double -stranded RNA. The complete genome is 11980 nucleotides long. Genome is sequenced, but only an estimate is available, complete sequence is 6350 nucleotides long.   Is sequenced, complete sequence is about 5630 nucleotides long.

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins. Virions consist of 1 structural protein(s).

Lipids

Lipids are not reported.

Antigenicity

Virions of the virus are closely serologically related to those of lettuce big vein virus, but as the two differ in host range, they are considered as distinct species, rather than related strains (Kuwata and Kubo, 1986).

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass ASTERIDAE; Order Solanales;
Family Solanaceae. Virus found in Nicotiana tabacum (common tobacco) [TaxID 4097].

General Symptoms in Plants Virus affects the photosynthetic system. Symptoms are expressed in the leaf and stem; include stunting; include rosetting; include deformation of internodes.

Severity and Occurrence of Disease

Host: Signs and symptoms vary seasonally.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by grafting; not transmitted by contact between hosts; not transmitted by seeds; not transmitted by pollen.

Vector Transmission:
Virus is transmitted by fungi; of the order Chytridiales; Olpidium brassicae. Virus does not require a helper virus for vector transmission.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Caryophyllaceae, Chenopodiaceae, Cucurbitaceae, Leguminosae-Papilionoideae, Solanaceae, Tetragoniaceae. The following species were susceptible to experimental virus infection: Beta vulgaris, Catharanthus roseus, Chenopodium album, Chenopodium amaranticolor, Chenopodium quinoa, Cucumis sativus, Cyamopsis tetragonoloba, Datura stramonium, Datura tatula, Dianthus caryophyllus, Glycine max, Gomphrena globosa, Lycopersicon esculentum, Nicotiana benthamiana, Nicotiana debneyi, Nicotiana glutinosa, Nicotiana megalosiphon, Nicotiana rustica, Nicotiana sylvestris, Nicotiana tabacum, Petunia x hybrida, Physalis floridana, Pisum sativum, Solanum tuberosum, Spinacia oleracea, Tetragonia tetragonioides.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Compositae, Cruciferae, or Cucurbitaceae, Gramineae, Leguminosae-Papilionoideae, Phytolaccaceae, Scrophulariaceae, Solanaceae, Umbelliferae. Species inoculated with virus that do not show signs of susceptibility: Antirrhinum majus, Apium graveolens, Arachis hypogaea, Brassica campestris ssp. napus, Brassica oleracea, Brassica oleracea var. botrytis, Brassica oleracea var. capitata, Calendula officinalis, Capsicum annuum, Celosia argentea, Cucurbita pepo, Cyamopsis tetragonoloba, Daucus carota, Lactuca sativa, Medicago sativa, Nicotiana bigelovii, Nicotiana clevelandii, Petroselinum crispum, Phaseolus vulgaris, Phytolacca americana, Raphanus sativus, Solanum demissum, Trifolium hybridum, Trifolium pratense, Triticum aestivum, Vicia faba, Zea mays, Zinnia elegans.

Diagnostic Hosts

Diagnostic host species and symptoms:

Chenopodium amaranticolor, Cyamopsis tetragonoloba, Nicotiana tabacum cv. Burley 21 — necrotic leaf spotting.

Nicotiana tabacum cv. Xanthi-nc — faint leaf spotting.

Tetragonia tetragonioides — yellow leaf spotting; not systemic.

Diagnostic host: insusceptible host species Brassica oleracea, Lactuca sativa, Capsicum annuum.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Nicotiana tabacum cvs Burley 21, Bright Yellow.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Chenopodium amaranticolor (L), Cyamopsis tetragonoloba (L), Nicotiana glutinosa (L,W), N. tabacum (L,W), Tetragonia tetragonioides (L).

References to host data: Hiruki (1975).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Cytopathology: Inclusions are not present in infected cells.

Geographical Distribution

The virus occurs in Japan.

References

Hidaka, Z. (1950). Ann. Phytopath. Soc. Japan 15: 40.

Hidaka, Z., Hiruki, C., Nakano, K., Shimizu, T. and Uozumi, T. (1956). Bull. Hatano Tobacco Exp. Stn 40: 1.

Hiruki, C. (1965). Virology 25: 288.

Hiruki, C. (1975). Can. J. Bot. 53: 2425.

Kuwata, S. and Kubo, S. (1981). Ann. Phytopath. Soc. Japan 47: 264.

Kuwata, S. and Kubo, S. (1986). AAB Descr. Pl. Viruses No. 313, 4 pp.

The following generic references are cited in the most recent ICTV Report.

PubMed References. A description of this taxon in VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 812 by S. Kuwata, 1985. Revised 1989.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 313.

Taxonomic Proposals and Changes

A taxonomic proposal has been submitted to the ICTV by the Plant Virus Subcommittee at the meeting in San Diego, March 1998 to include a new taxon (in to the newly created genus Varicosavirus). The proposal has been approved at the meeting of the Executive Committee in San Diego, 1998, the taxon has been designated as Species.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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