[Home] [ICTV Taxonomy - Index of Viruses] [Virus Descriptions] [Character List] [Picture Gallery]
[Tutorial] [Online Data Retrieval & Identification] [Virus Isolate Registration & Submission] [Search]
Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.074.0.02.012. Turnip crinkle virus

Cite this publication as: ICTVdB Management (2006). 00.074.0.02.012. Turnip crinkle virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Scotland; the United Kingdom.

Host of Isolate and Habitat Details
Source of isolate: Brassica campestris ssp. rapa.

Natural host and symptoms
Brassica campestris ssp. rapa — mottling and leaf distortion; stunting of plants.

Brassica napus var. napobrassica — faint leaf chlorosis or symptomless infection.

Brassica sinapis — severe leaf distortion.

Reference to Isolation Report
Broadbent and Blencowe (1955).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.074.0.02.012. Virus accession number: 74002012. Obsolete virus code: 74.0.2.0.012; superceded accession number: 74020012.
NCBI Taxon Identifier NCBI Taxonomy ID: 11988.

Name, Synonyms and Lineage

ICTV approved acronym: TCV. Virus is an ICTV approved species of the genus 00.074.0.02. Carmovirus in the family 00.074. Tombusviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry (T=3). The capsid is isometric and has a diameter of 28 nm. Capsids appear round. The capsid surface structure reveals a regular pattern with distinctive features (of granular appearance). The capsomer arrangement is clearly visible. The capsid consists of 32 capsomers (virion composed of 180 protein subunits).

Electron microscopic preparation and references: Reference for electron microscopic methods: Hollings and Stone (1969).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.34 g cm-3. There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 129 S20w. A260/A280 ratio is 1.48. The thermal inactivation point (TIP) is at 95°C. The longevity in vitro (LIV) is more than 42 days (at 20°C). Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 6.

Nucleic Acid

The Mr of the genome constitutes 17% of the virion by weight. The genome is monopartite. Only one particle size of linear, positive-sense, single-stranded RNA is recovered. The complete genome is 4051 nucleotides long, is fully sequenced, complete sequence is 4051 nucleotides long. Sequenced genome has the accession number(s)
[M22445] Em(40)_vi:TCVPORF Gb(84)_vi:TCVPORF Turnip crinkle virus P28, P88, P8 and coat protein genes RNA, complete cds. 11/89 4,050bp
[M29290] Em(40)_vi:TCVDIGAA Gb(84)_vi:TCVDIGAA Turnip crinkle virus defective interfering RNA. 5/90 347bp.
[X05193] Em(40)_vi:TOTCVCP Gb(84)_vi:TOTCVCP Turnip crinkle virus RNA for coat protein. 9/93 1,450bp.
[X12749] Em(40)_vi:TOTCVRF Gb(84)_vi:TOTCVRF Turnip crinkle virus (TCV) avirulent satellite RNA F. 12/89 230bp.
[X12750] Em(40)_vi:TOTCVRC Gb(84)_vi:TOTCVRC Turnip crinkle virus (TCV) virulent satellite RNA C. 4/89 355bp. 5 sequences.

The genome has no intergenic poly (A) region. Each virion contains a longer than full length copy. Reference to nucleotide sequence Carrington et al. (1987).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

Proteins constitute about 83% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 1 structural protein(s) located in the capsid.

Structural Proteins: Capsid protein is the coat protein.

Reference to amino acid sequence or composition Carrington (1986, Carrington et al. (1987, Butler (1970).

Lipids

Lipids are not reported.

Antigenicity

The virus is serologically related to galinsoga mosaic virus. Suggested relationship to carnation mottle virus not yet confirmed. The virus does not show serological relationships to carnation mottle virus.

The virus showed no RNA sequence homology with galinsoga mosaic, cucumber soil-borne, saguaro cactus or Pelargonium flower break viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Class Magnoliopsida (Dicotyledonae)
Subclass DILLENIIDAE.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by contact between hosts; not transmitted by seeds.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Coleoptera; Phyllotreta (nine species) and Psylliodes (two species). Virus is transmitted in a non-persistent manner.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in many families. Susceptible host species are found in the Family Amaranthaceae, Apocynaceae, Chenopodiaceae, Compositae, Cruciferae, Cucurbitaceae, Solanaceae, Tetragoniaceae. The following species were susceptible to experimental virus infection: Brassica campestris ssp. napus, Brassica campestris ssp. pekinensis, Brassica campestris ssp. rapa, Brassica juncea, Brassica napus var. napobrassica, Brassica oleracea var. botrytis, Brassica oleracea var. capitata, Brassica sinapis, Capsella bursa-pastoris, Catharanthus roseus, Cheiranthus cheiri, Chenopodium album, Chenopodium amaranticolor, Chenopodium murale, Chenopodium quinoa, Cucumis sativus, Datura stramonium, Gomphrena globosa, Lactuca sativa, Lunaria annua, Lycopersicon esculentum, Nicotiana bigelovii, Raphanus sativus, Sinapis alba, Tetragonia tetragonioides.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Chenopodiaceae, or Leguminosae-Papilionoideae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Amaranthus caudatus, Beta vulgaris, Nicotiana clevelandii, Nicotiana tabacum, Phaseolus vulgaris, Vicia faba.

Diagnostic Hosts

Diagnostic host species and symptoms:

Brassica campestris ssp. pekinensis — local chlorotic lesions; systemic leaf mottling and crinkling.

Chenopodium amaranticolor, C. quinoa, Datura stramonium — local chlorotic lesions; no systemic infection.

Chenopodium murale — local chlorotic lesions; systemic chlorotic flecking.

Tetragonia tetragonioides — local chlorotic lesions; symptomless systemic infection.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Brassica juncea, Brassica campestris ssp. pekinensis.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Brassica juncea (W), Brassica campestris ssp. pekinensis (W), Chenopodium amaranticolor (L), C. murale (L), C. quinoa (L), Tetragonia tetragonioides (L).

References to host data: Broadbent and Heathcote (1958).

Histopathology: Virus can be best detected in all parts of the host plant.

Cytopathology: Inclusions are not present in infected cells.

Geographical Distribution

The virus occurs in the United Kingdom and Yugoslavia.

References

Altenbach, S.B. and Howell, S.H. (1982). Virology 118: 128.

Broadbent, L. and Blencowe, J.W. (1955). Rep. Rothamsted Exp. Stn. 1954, p. 87.

Broadbent, L. and Heathcote, GD (1958). Ann. appl. Biol. 46: 585.

Butler, P.JG (1970). J. mol. Biol. 52: 589.

Carrington, JC (1986). Ph.D. Thesis. University of California, Berkeley, U.S.A.

Carrington, J.C., Heaton, L.A., Zuidema, D., Hillman, B.I. and Morris, TJ (1989). Virology 170: 219.

Carrington, J.C., Morris, TJ, Stockley, P.G. and Harrison, S.C. (1987). J. mol. Biol. 194: 265.

Dougherty, WG and Kaesberg, P. (1981). Virology 115: 45.

Finch, JT, Klug, A and Leberman, R. (1970). J. mol. Biol. 50: 215.

Hacker, DL, Petty, I.R.D., Wei, N. and Morris, TJ (1992). Virology 186: 1.

Hollings, M. and Stone, O.M. (1969). Zbl. Bakt. ParasitKde. Abt. 2, 123: 237.

Leberman, R. and Finch, JT (1970). J. mol. Biol. 50: 209.

McLeod, R. and Markham, R. (1963). Virology 19: 190.

Martin, C. (1958). Proc. 3rd Conf. Potato Virus Dis., Lisse-Wageningen, 1957, p. 106.

Symons, RH, Rees, M.W., Short, M.N. and Markham, R. (1963). J. mol. Biol. 6: 1.

Tremaine, J. (1970). Virology 42: 611.
The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 854 by A.A. Brunt, 1991.



Limit search to: Title & Body Title Document Path
Show Reverse Sort

DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


Additional access points to virus species lists, descriptions and images on the web:

Species catalogue                     iSpecies.org - a species search engine           a species search engine

Google Analytics      Google Analytics: activity view