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00.067.0.81.014. Ginger chlorotic fleck virus

Cite this publication as: ICTVdB Management (2006). 00.067.0.81.014. Ginger chlorotic fleck virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Queensland; Australia (and Neu Zealand).

Host of Isolate and Habitat Details
Source of isolate: Zingiber officinale.

Natural host and symptoms
Zingiber officinale — chlorotic leaf flecking.

Reference to Isolation Report
Thomas (1986).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.067.0.81.014. Virus accession number: 67081014. Obsolete virus code: 67.0.1.T.014; superceded accession number: 6701t014.

Name, Synonyms and Lineage

ICTV approved acronym: GCFV. Virus is a tentative member of the genus 00.067.0.01. Sobemovirus; not assigned to a family.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 28-33 nm (from side to side, 4 nm less than from vertex to vertex). Capsids appear hexagonal in outline. The capsomer arrangement is not obvious.

Electron microscopic preparation and references: Virus preparation contains many virions. Virions are unstable in PTA, but stable in UA and AM. Reference for electron microscopic methods: Thomas (1986).

Physicochemical and Physical Properties

Virions have a buoyant density in CsCl of 1.355 g cm-3 (fixed). The density of virions is in Cs2SO4 1.297 g cm-3 at pH 4.9 and 1.293 g cm-3 at pH 6.9. There are 1 sedimenting component(s) found in purified preparations. The sedimentation coefficient is 111 S20w (in 10 mM phosphate pH7, 20°C).

Nucleic Acid

The Mr of the genome constitutes 20% of the virion by weight. The genome is monopartite. Only one particle size of linear, positive-sense, single-stranded RNA is recovered. Minor species of genomic nucleic acid are found in addition to the full length genome; there are 2 minor species found. The complete genome is 4200 nucleotides long, is sequenced, complete sequence is about 4200 nucleotides long. Reference to nucleotide sequence Thomas (1986).

Proteins

Proteins constitute about 80% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins. Virions consist of 2 structural protein(s) (the smaller probably a degradation product of the larger).

Structural Proteins: Reference to method of preparation: Thomas (1986): SDS digestion.

Lipids

Lipids are not reported.

Antigenicity

The virus does not show serological relationships to carnation mottle, cocksfoot mild mottle (syn. phleum mottle), cocksfoot mottle, cucumber leaf spot (cucumber fruit streak strain), cucumber mosaic, Cymbidium ringspot, elm latent, galinsoga mosaic, glycine yellow mosaic, hibiscus chlorotic ringspot, lucerne transient streak, narcissus tip necrosis, Pelargonium flower break, Pelargonium line pattern, Pelargonium vein netting, red clover necrotic mosaic, saguaro cactus, solanum nodiflorum mottle, southern bean mosaic, sowbane mosaic, tobacco necrosis, turnip crinkle, turnip rosette and velvet tobacco mottle viruses.

Diagnostics and Reference Collections

The best tests for diagnosis are easily diagnosed by serological methods.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by mechanical inoculation.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Zingiberaceae. The following species were susceptible to experimental virus infection: Zingiber officinale.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Chenopodiaceae, or Cucurbitaceae, Gramineae, Leguminosae-Papilionoideae, Musaceae, or Solanaceae, Zingiberaceae. Species inoculated with virus that do not show signs of susceptibility: Chenopodium amaranticolor, Chenopodium quinoa, Cucumis sativus, Elettaria cardamomum, Gomphrena globosa, Hedychium gardnerianum, Musa acuminata (cv. Cavendish), Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Sorghum bicolor, Vigna unguiculata, Zea mays.

Diagnostic Hosts

Diagnostic host species and symptoms:

Zingiber officinale — systemic chlorotic flecking.

Diagnostic host: insusceptible host species Gomphrena globosa, Chenopodium quinoa, Cucumis sativus cv. Supermarket, Phaseolus vulgaris cv. Bountiful, Zea mays cv. Iochief.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Zingiber officinale.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Zingiber officinale (W). No local lesion host.

References to host data: Thomas (1986).

Histopathology: Virus can be best detected in leaves and mesophyll. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are membranous bodies.

Geographical Distribution

The virus is found, but with no evidence of proliferation, in Australia, where it was isolated from ginger imported from India, Malaysia, Mauritius and Thailand, but all infected material was destroyed and there was no evidence that it became established in Australia. Its presence has not yet been confirmed in the countries of origin of the infected ginger.

References

Thomas, JE (1986). Ann. appl. Biol. 108: 43.

Thomas, JE (1988). AAB Descr. Pl. Viruses No. 328, 4 pp.

The following generic references are cited in the most recent ICTV Report.
VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 356 by J.E. Thomas, 1984. Revised 1987.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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