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00.060.0.07.005. Oat sterile dwarf virus

Cite this publication as: ICTVdB Management (2006). 00.060.0.07.005. Oat sterile dwarf virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: Czechoslovakia (former) (and Sweden).

Host of Isolate and Habitat Details
Source of isolate: Avena sativa.

Natural host and symptoms
Arrhenatherum elatius, Avena sativa, Hordeum vulgare, Lolium multiflorum, L. perenne — dwarfing, excess tillering, flower suppression and retention of non-maturing juvenile phase; dark green colour of leaves, which may be notched, ragged or malformed; small enations on abaxial surfaces of leaves, nodes and spike.

Reference to Isolation Report
Prusa (1958, Lindsten (1959).

Classification

This is a description of a plant virus at the species level with data on all virus properties from morphology to genome, replication, antigenicity and biological properties.

ICTVdB Virus Code: 00.060.0.07.005. Virus accession number: 60007005. Obsolete virus code: 60.0.7.3.001; superceded accession number: 60073001.
NCBI Taxon Identifier NCBI Taxonomy ID: 73147.

Name, Synonyms and Lineage

Synonym(s): Arrhenatherum blue dwarf virus (Milne and Lesemann, 1978; Muhle and Kempiak, 1971; Luisoni et al., 1979), Lolium enation virus (Lesemann and Huth, 1975; Milne and Lesemann, 1978). ICTV approved acronym: OSDV. Virus is an ICTV approved species of the genus 00.060.0.07. Fijivirus; family 00.060. Reoviridae.

Virion Properties

Morphology

Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is isometric with icosahedral symmetry and has a diameter of 65-70 nm (complete virions). The capsid shells of virions are composed of two layers. All shells are usually present. Capsids appear round. Inner capsids consist of a 55 nm. The core is spherical.

Electron microscopic preparation and references: Virus preparation contains few virions. Take material from enations only, or swollen leaf veins; use UA, not PTA, glutaraldehyde causes damage. Reference for electron microscopic methods: Luisoni et al. (1979, Boccardo and Milne (1980). Only B-spiked subvirions (SVPs) have been purified. Use 0.4M phosphate buffer pH 7, shake with Freon, recover SVPs in aqueous phase; apply 2 cycles of differential centrifugation, and a final rate-zonal caesium sulphate density gradient.

Physicochemical and Physical Properties

There are 1-4 sedimenting component(s) found in purified preparations.

Nucleic Acid

The genome is segmented and consists of ten segments of linear double-stranded RNA. The complete genome is 28910 nucleotides long, is sequenced and complete sequence is about 4400 nucleotides long, is sequenced, but only an estimate is available, complete sequence is 3900 nucleotides long.   Is sequenced, but only an estimate is given, complete sequence is 3700 nucleotides long, has been sequenced, but only an estimate is provided; complete sequence is 3700 nucleotides long and has been sequenced, but only an estimate is presented, complete sequence is 3300 nucleotides long. RNA-6 has been fully sequenced, complete sequence is 2900 nucleotides long. RNA-7 has been sequenced, but only an estimate is presented, complete sequence is 1860 nucleotides long. RNA-8 has been sequenced and complete sequence is about 1830 nucleotides long. RNA-9 has been sequenced, but only an estimate is available, complete sequence is 1820 nucleotides long. RNA-10 has been sequenced, but only an estimate is available; complete sequence is 1500 nucleotides long. Reference to nucleotide sequence Luisoni et al. (1979).

GenBank records for nucleotide sequences; complete genome sequences.

Proteins

The viral genome encodes structural proteins and non-structural proteins.

Lipids

Lipids are absent.

Translation: The genome replicates in the cytoplasm (of phloem and related cells of plants, and in the cytoplasm of many cell types in insects).

Virions are associated with helper virus, but independent from its functions during replication.

Antigenicity

The virus does not show serological relationships to sugarcane fiji disease, maize rough dwarf, rice black-streaked dwarf and pangola stunt viruses.

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Liliopsida (Monocotyledonae).

General Symptoms in Plants Symptoms bushy, dwarf, dark green grass-like appearance; leaf malformation, yellowish or white enations (small galls) centred on veins or vein swellings on backs of leaves.

Severity and Occurrence of Disease

Host: Signs and symptoms persist.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; not transmitted by contact between hosts; not transmitted by seeds; not transmitted by pollen.

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Hemiptera, family Delphacidae; Javesella pellucida, J. dubia, J. discolor, J. obscurella, Dicranotropis hamata. The principal natural vector(s) are Javesella pellucida. Virus is not transmitted by Laodelphax striatellus. Virus is transmitted in a persistent manner; retained when the vector moults; replicates in the vector; does not require a helper virus for vector transmission.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in few families. Susceptible host species are found in the Family Gramineae. The following species were susceptible to experimental virus infection: Arrhenatherum elatius, Avena fatua, Avena sativa, Cynosurus cristatus, Festuca pratensis, Holcus lanatus, Hordeum vulgare, Lagurus ovatus, Lolium multiflorum, Lolium perenne, Lolium temulentum, Phalaris canariensis, Poa annua, Secale cereale, Triticum aestivum, Zea mays.

Host:
Experimentally infected hosts mainly show symptoms of bushy, dwarf, dark green grass-like appearance; leaf malformation, yellow or white enations on veins or vein swellings on backs of leaves.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Gramineae. Species inoculated with virus that do not show signs of susceptibility: Agrostis gigantea, Alopecurus myosuroides, Apera spica-venti, Bromus inermis, Digitaria sanguinalis, Echinochloa crus-galli, Elytrigia repens.

Diagnostic Hosts

Diagnostic host species and symptoms:

Arrhenatherum elatius, Avena ssp., Cynosurus cristatus, Hordeum ssp., Lolium ssp., Phalaris canariensis, Poa annua, Secale cereale, Triticum ssp., Zea ssp. — bushy dwarf dark green grasslike appearance, leaf malformation, yellow or white enations on veins or vein swellings on backs of leaves. Diagnostic host: insusceptible host species all species except those from the Gramineae. Insusceptible Gramineae include Elytrigia repens, Agrostis gigantea, Bromus inermis, Digitaria sanguinalis, Echinochloa crus-galli.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Lolium multiflorum.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Avena sativa (W), Lolium multiflorum (W).

References to host data: Milne and Lovisolo (1977).

Histopathology: Virus can be best detected in leaves, roots, phloem and companion cells. Virions are found in the cytoplasm (of veins of infected plants are swollen or develop enations consisting of enlarged and proliferated phloem parenchyma. Most virions are found in these cells, and in the roots).

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Cytoplasmic inclusions are viroplasma. Inclusions are crystalline arrays of complete virions and tubules or scroll-like structures composed of protein, usually containing virions. Inclusions contain mature virions. Other cellular changes include cell proliferation (Milne and Lovisolo, 1977; Milne, 1980).

Geographical Distribution

The virus occurs in Czechoslovakia (former), Finland, Germany, Norway, Poland, Sweden, and the United Kingdom.

Ecology, Epidemiology and Control

Studies reported by Boccardo and Milne (1980, Lindsten (1979, BrcHak (1979).

References

Boccardo, G. and Milne, R.G. (1980). CMI/AAB Descr. Pl. Viruses No. 217.

Boccardo, G. and Milne, R.G. (1984). CMI/AAB Descr. Pl. Viruses No. 294, 7pp.

BrcHak, J. (1979). In: Leafhopper Vectors and Plant Disease Agents p. 97; eds K. Maramorosch and K. Harris. Academic Press, New York.

Lesemann, D.-E. and Huth, W. (1975). Phytopath. Z. 82: 246.

Lindsten, K. (1959). Phytopath. Z. 35: 420.

Lindsten, K. (1979). In: Leafhopper Vectors and Plant Disease Agents. p. 155; eds K. Maramorosch and K. Harris, Academic Press, New York.

Luisoni, E., Boccardo, G., Milne, R.G. and Conti, M. (1979). J. gen. Virol. 45: 651.

Milne, R.G. (1980). Microbiologica 3: 333.

Milne, R.G. and Lesemann, D.-E. (1978). Virology 90: 299.

Milne, R.G. and Lovisolo, O. (1977). Adv. Virus Res. 21: 267.

Milne, R.G. and Luisoni, E. (1977). Virology 80: 12.

Milne, R.G., Lindsten, K. and Conti, M. (1975). Ann. appl. Biol. 79: 371.

Muhle, E. and Kempiak, G. (1971). Phytopath. Z. 72: 269.

Prusa, V. (1958). Phytopath. Z. 33: 99.

The following generic references are cited in the most recent ICTV Report.

PubMed References.

VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 530 by R.G. Milne, 1991. A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 217.

Images

Taxon images: • EM from IACR Rothamsted.



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ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

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