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Descriptions are generated automatically from the ICTVdB database including links. Some descriptions are only very basic and links may point to documents that are not yet published on the Web.

00.060.0.01.004. Mammalian orthoreovirus

Cite this publication as: ICTVdB Management (2006). 00.060.0.01.004. Mammalian orthoreovirus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Classification

This is a description of a vertebrate virus at the species level.

ICTVdB Virus Code: 00.060.0.01.004. Virus accession number: 60001004. Obsolete virus code: 60.0.1.1.; superceded accession number: 60011000.

Name, Synonyms and Lineage

Virus is an ICTV approved species of the genus 00.060.0.01. Orthoreovirus; family 00.060. Reoviridae.

Virion Properties

Morphology

Virions consist of a capsid, a core, and a nucleoprotein complex. Virus capsid is not enveloped. Capsid/nucleocapsid is isometric with icosahedral symmetry and has a diameter of 80-82 nm. The capsid shells of virions are composed of two layers. All shells are usually present, or the outer shell is often lost during preparation. Capsids appear round. The capsid surface structure reveals a regular pattern with distinctive features. The capsomer arrangement is clearly visible. Surface projections are not present. Inner capsids core (T = 1) have a diameter of 70.6-75.6 nm. Virus preparations contain one particle component. The core is spherical.

Only one species is recovered in preparations. Incomplete particles are common. They are devoid of nucleic acid characterized by capsids with dark centers in negative stain preparations, or incompletely assembled capsids.



























Movies

QuickTime: MOV, .QT
MPEG: MPG		 (1477 Kb | MOV) (1366 Kb | MPG) (2017 Kb | MOV) (316 Kb | MOV) (203 Kb | MPG) (1028 Kb | MOV) (410 Kb | MPG)

Notes

 Mammalian Reovirus Virion
as solved by Cryoelectron Microscopy and Image Reconstuction.
The cryoEM data was from Tim Baker's Laborratory, Purdue University. Movies were created by Stephan Spencer

Animations of the virion of mammalian reovirus, as solved by cryo-electron microscopy and image reconstruction. Rendered as an isosurface using Iris Explorer on a Silicon Graphics workstation.

Isosurfaces are used to portray the three-dimensional structure of the virus. An isosurface is obtained by converting the subset of electron density values in the three-dimensional array that contours a chosen threshold value (isovalue) into a surface representation approximated by polygons.

Structural elements of interest include the five-fold vertices (five-fold symmetrical stars) containing lambda-2 protein and the hexameric and tetrameric flower-like projections composed of sigma-3 protein.

Annimation of reconstructed viruses are found at Virus World, Molecular Virology, Madison.

Nucleic Acid

The Mr of the genome constitutes 15-20% of the virion by weight. The genome is monomeric; segmented and consists of ten segments of linear double-stranded RNA. The complete genome is 23700 nucleotides long. The L1 is fully sequenced, complete sequence is 3854 nucleotides long. L2 is fully sequenced, complete sequence is 3916 nucleotides long. L3 is fully sequenced, complete sequence is 1200 nucleotides long. M1 has been fully sequenced. Complete sequence is 2200-2300 nucleotides long. M2 has been sequenced, but only an estimate is presented, complete sequence is 2210 nucleotides long. M3 has been sequenced, but only an estimate is given, complete sequence is 2235 nucleotides long, has been sequenced, but only an estimate is presented; complete sequence is 1420 nucleotides long. S2 has been fully sequenced, complete sequence is 1330 nucleotides long. S3 has been is about 1200-1400 nucleotides long. S4; complete sequence is 1200-1400 nucleotides long. The 5'-end of the genome has a methylated nucleotide cap; cap sequence type is m7G5ppp5'GmpNp. The multipartite genome is found in one type of particle only. Each virion contains a single copy of the genome.

Proteins

Proteins constitute about 80-85% of the particle weight.

The viral genome encodes structural proteins and non-structural proteins located in the capsid, inner capsid, and core.

Structural Proteins: Inner capsid protein lambda 1 (gene name L3), 60 subunits; has a molecular mass of 137353 Da (1233 AA, sequence has the accession number [P15024]; is a major core protein. Inner capsid protein mu-2 (gene name M1), subunits 60 (L2, has a molecular mass of 83276 Da (736 AA, sequence has the accession number [P12418]; is a minor structural protein. Inner capsid or core protein, sigma 2 (gene name S2), with subunits 150 (S2) has a molecular weight of 47161, is 418 AA long and has the accession number [P03525].

Lipids

Lipids are absent.

Biological Properties

Natural Host

Virus infects during its life cycle a single type of vertebrate host.
Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Animalia.

Kingdom Animalia
Phylum Chordata.

Phylum Vertebrata
Subphylum Vertebrata.

Class Mammalia.

Transmission and Vector Relationships

Virus is transmitted by a vector in a direct manner.

List of Strains and Isolates in the Species 00.060.0.01.004.01.001.001. Reovirus 1 (strain Lang, 00.060.0.01.004.01.002.001. Reovirus 2 (strain D5/Jones, 00.060.0.01.004.01.003. Reovirus 3 strain Dearing.

References

The following generic references are cited in the most recent ICTV Report.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

ICTVdB and DELTA related References

Comments to ICTVdB Management
Last updated on 25 April 2006 by Cornelia Büchen-Osmond
Copyright © 2002    International Committee on Taxonomy of Viruses.    All rights reserved.


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