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00.018.0.01.005. Broad bean stain virus

Cite this publication as: ICTVdB Management (2006). 00.018.0.01.005. Broad bean stain virus. In: ICTVdB - The Universal Virus Database, version 4. Büchen-Osmond, C. (Ed), Columbia University, New York, USA

Cite this site as: ICTVdB - The Universal Virus Database, version 4. http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/

Table of Contents

Isolate Description

Location: the United Kingdom.

Host of Isolate and Habitat Details
Source of isolate: Vicia faba.

Natural host and symptoms
Vicia faba — systemic mottling, necrosis and malformation.

Reference to Isolation Report
Lloyd et al. (1965).

Classification

This is a description of a plant virus at the species level.

ICTVdB Virus Code: 00.018.0.01.005. Virus accession number: 18001005. Obsolete virus code: 18.0.1.0.005; superceded accession number: 18010005.

Name, Synonyms and Lineage

Synonym(s): virus de la mosaique de la fSve, MF virus, broad bean F1 virus (Gibbs et al., 1968), broad bean Evesham stain virus. ICTV approved acronym: BBSV. Virus is an ICTV approved species of the genus 00.018.0.01. Comovirus in the family 00.018. Comoviridae.

Virion Properties

Morphology

Virions consist of a capsid. Virus capsid is not enveloped, round with icosahedral symmetry. The isometric capsid has a diameter of 28 nm. Capsids appear hexagonal in outline. The capsomer arrangement is not obvious.

Electron microscopic preparation and references: Virus preparation contains many virions. Reference for electron microscopic methods: Gibbs et al. (1968).

Physicochemical and Physical Properties

There are 3 sedimenting component(s) found in purified preparations. The sedimentation coefficient is of the fastest 127 S20w (B, of the other(s) are 100 S20w (M), or 60 S20w (T). The thermal inactivation point (TIP) is at 60-65°C. The longevity in vitro (LIV) is 31 days. Although the titer is dependent on the host, the decimal exponent (DEX) of the dilution end point is usually around 3. The infectivity is not changed by treatment with ether; retained when deproteinized with phenol or detergent.

Nucleic Acid

The Mr of the genome constitutes 34% of the virion by weight (B), or 25% of the virion by weight (M), or 0% of the virion by weight (T). The genome is segmented; bipartite, segements are distributed among 2 particle types of linear, positive-sense, single-stranded RNA. The complete genome is 11250 nucleotides long. Genome is sequenced, but only an estimate is available, complete sequence is 6750 nucleotides long.   Is sequenced, complete sequence is about 4500 nucleotides long. The genome has a base ratio of 23.3 % guanine; 26.5 % adenine; 18.4 % cytosine; 31.9 % uracil. The multipartite genome is divided among different particles, each segment encapsidated separately and the segments are distributed between 2 different types of particles. The largest particles contain each one molecule of RNA-1 (sedimenting component B). The medium sized particles contain each one molecule of RNA-2 (sedimenting component M). The smallest particles contain no nucleic acid and are found in the top (sedimenting component T) band after sedimentation.

Proteins

Proteins constitute about 66% of the particle weight (T), or 75% of the particle weight (M), or 100% of the particle weight (T).

The viral genome encodes structural proteins and non-structural proteins.

Lipids

Lipids are not reported.

Antigenicity

The virus is serologically related to cowpea mosaic, cowpea severe mosaic, glycine mosaic, red clover mottle and squash mosaic viruses, but distantly (Beczner et al., 1983).

Biological Properties

Natural Host

Domain
Viral hosts belong to the Domain Eucarya.

Domain Eucarya
Kingdom Plantae.

Kingdom Plantae
Phylum Magnoliophyta (Angiosperms, Class Magnoliopsida (Dicotyledonae).

Severity and Occurrence of Disease

Host: Signs and symptoms vary cyclically over a few weeks, or vary seasonally.

Transmission and Vector Relationships

Virus is transmitted by a vector. Virus is transmitted by mechanical inoculation; transmitted by grafting; transmitted by seeds (up to 10% in some Vicia faba cultivars).

Vector Transmission:
Virus is transmitted by arthropods, by insects of the order Coleoptera; Apion vorax, Sitona ssp.

Experimental Hosts and Symptoms

Under experimental conditions susceptibility to infection by virus is found in several families. Susceptible host species are found in the Family Chenopodiaceae, Leguminosae-Papilionoideae, Solanaceae. The following species were susceptible to experimental virus infection: Chenopodium album, Chenopodium capitatum, Chenopodium murale, Chenopodium quinoa, Cicer arietinum, Glycine max, Lathyrus odoratus, Melilotus albus, Nicotiana debneyi, Phaseolus vulgaris, Pisum sativum, Trifolium hybridum, Trifolium incarnatum, Vicia faba, Vigna unguiculata.

Host:
Experimentally infected hosts mainly show symptoms of mottle, mosaic, necrosis.

Experimentally infected insusceptible Hosts: Families containing insusceptible hosts: Amaranthaceae, Chenopodiaceae, or Leguminosae-Caesalpinioideae, Leguminosae-Papilionoideae, Solanaceae. Species inoculated with virus that do not show signs of susceptibility: Cassia occidentalis, Chenopodium amaranticolor, Cyamopsis tetragonoloba, Datura stramonium, Gomphrena globosa, Lens culinaris, Lotus corniculatus, Medicago sativa, Nicotiana clevelandii, Nicotiana glutinosa, Nicotiana megalosiphon, Nicotiana rustica, Nicotiana sylvestris, Nicotiana tabacum, Phaseolus vulgaris (cv. Pinto), Trifolium pratense, Trifolium repens, Vigna radiata.

Diagnostic Hosts

Diagnostic host species and symptoms:

Phaseolus vulgaris cv. Tendergreen — systemic mottle.

Pisum sativum, Vicia faba — systemic mottle; necrosis.

Diagnostic host: insusceptible host species Chenopodium amaranticolor, Phaseolus vulgaris cv. Pinto, Nicotiana clevelandii.

Maintenance and Propagation Hosts

Most commonly used maintenance and propagation host species are Phaseolus vulgaris cv. Tendergreen, Pisum sativum, Vicia faba.

Assay Hosts

Host: Assay hosts (for Local lesions or Whole plants):
Phaseolus vulgaris cv. Tendergreen (L), Vicia faba (W).

References to host data: Thornberry (1966, Moghal and Francki (1974, Musil et al. (1978, Valenta et al. (1979).

Histopathology: Virus can be best detected in all parts of the host plant. Virions are found in the cytoplasm.

Cytopathology: Inclusions are present in infected cells. Inclusion bodies in the host cell are found in the cytoplasm. Inclusions are rows of virions in tubules, finger-like outgrowths at plasmodesmata, vacuolate-vesiculate bodies and virus aggregates (Russo et al., 1982).

Geographical Distribution

The virus spreads in Africa and Eurasia. The virus is found, but with no evidence of proliferation, in Australia from imported seed.

List of Strains and Isolates in the Species

Pea green mottle virus.

References

Beczner, L., Musil, M. and Kowalska, C. (1983). Zesz. probl. Poste. Nauk rolm. 291: 21.

Cockbain, AJ., Cook, SM and Bowen, R.(1975). Ann. appl. Biol. 81: 331.

Gibbs, AJ. and Smith, H.G. (1970). CMI/AAB Descr. Pl. Viruses No. 29, 3 pp.

Gibbs, AJ., Giussani-Belli, G. and Smith, H.G. (1968). Ann. appl. Biol. 61: 99.

Jones, AT and Barker, H. (1976). Ann. appl. Biol. 83: 231.

Lloyd, ATE., Smith, H.G. and Jones, L.H. (1965). Hort. Res. 5: 13.

Moghal, SM and Francki, RIB. (1974). Aust. J. biol. Sci. 27: 341.

Musil, M., Leskova, O. and Kleja, S. (1978). Ochr. Rost. 14, 161.

Russo, M., Castellano, MA and Martelli, G.P. (1982). J. Submicrosc. Cytol. 14: 149.

Valenta, V., Gressnerova, M., Marcinka, K. and Nermut, M.V. (1979). Acta Virol., Prague 13: 422. The following generic references are cited in the most recent ICTV Report.
VIDEdB, the plant virus database developed at the Australian National University by Adrian J. Gibbs and collaborators, contains an earlier description with the number 112 by A.J. Gibbs, 1981. Revised 1987.

A description of the virus is found in DPV, a database for plant viruses developed by the Association of Applied Biologists (AAB), with the number 29.



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DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia. ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses by Dr Cornelia Büchen-Osmond is written in DELTA. The virus descriptions in ICTVdB are coded by, or using data from experts in the field of virology or members ICTV. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations are automatically generated and formatted for display on the Web from the descriptions in DELTA-format. The description has been generated automatically from DELTA files. DELTA - DEscription Language for TAxonomy developed by Dr Mike Dallwitz, Toni Paine and Eric Zurcher, CSIRO Entomology, Canberra, Australia.

ICTVdB - The Universal Virus Database, developed for the International Committee on Taxonomy of Viruses (ICTV) by Dr Cornelia Büchen-Osmond, is written in DELTA. The virus descriptions in ICTVdB are coded by ICTV members and experts, or by the ICTVdB Management using data provided by the experts, the literature or the latest ICTV Report. The character list is the underlying code. All virus descriptions are based on the character list and natural language translations from the encoded descriptions are automatically generated and formatted for display on the Web.

Developer of the DELTA software: M. J. Dallwitz, T. Paine and E. Zurcher

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Last updated on 25 April 2006 by Cornelia Büchen-Osmond
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